Protein Assays
TOPOGEN INC Topoisomerase I Assay Kit 100 Assays Includes Top1
This kit contains reagents for routine detection of type I topoisomerase activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in order to prevent nucleolytic nicking of the supercoiled substrate (although we note that some eukaryotic type I activities are stimulated by Mg++). This assay will work even with crude extracts.
Enzo Life Sciences PROTEOSTAT Protein aggregation assay (2x96tests)
Quantitative Detection of Protein Aggregates from Visible to Subvisible Particles. PROTEOSTAT.0.5). Lyophilized native and aggregated protein are provided as negative and positive controls for monitoring changes in protein aggregation status.PROTEOSTAT.Easy to use - simply add water!. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, Microplate. Contents: PROTEOSTAT detection reagent. PROTEOSTAT positive control. PROTEOSTAT negative control. 10X PROTEOSTAT assay buffer
TOPOGEN INC Topoisomerase I Assay Kit 100 Assays
This kit contains reagents for routine detection of type I topoisomerase activity. DNA markers (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in order to prevent nucleolytic nicking of the supercoiled substrate (although we note that some eukaryotic type I activities are stimulated by Mg++). This assay will work even with crude extracts.
LICOR Revert™ 700 Total Protein Stain and Wash Solution Kit (1 kit)
Revert 700 Total Protein Stain is a robust and replicable solution for Western blot normalization. The stain can be detected in the 700nm channel of Odyssey Imaging Systems for quantification and normalization analyses. The protocol is quick and can be completed within 15 minutes. This kit contains 100 mL Revert 700 Total Protein Stain and 200 mL Revert 700 Wash Solution. This kit is sufficient for 20 mini Western blots.
Abcam MMP Activity Assay Kit (Fluorometric - Green)
MMP Activity Assay Kit (Fluorometric - Green)
The product is subject to the following: Abcam Restricted Use Statement
BPS Bioscience Inc ARE Reporter Kit (Nrf2 Antioxidant Pathway), 500 reactions
The ARE Reporter kit is designed for monitoring the activity of the Nrf2 antioxidant pathway in cultured cells. The kit contains a transfection-ready ARE luciferase reporter vector, which is an Nrf2 pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ARE responsive elements located upstream of a minimal promoter. The ARE reporter is premixed with a constitutively expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. This negative control is critical to determining pathway-specific effects and background luciferase activity.
AURORA BIOLABS LLC TEV PROTEASE Ready-to-Use, 10,000 Units
Tobacco Etch Virus nuclear-inclusion-a endopeptidase or TEV is highly sequence specific cysteine protease. It has a strict 7 amino acid cleavage recognition sequence of Glu-Asn-Leu-Tyr-Phe-Gln ↓ (Gly/Ser). The high specificity makes this protease excellent for the removal of affinity-tags from purified recombinant proteins. Purified TEV was expressed in E.coli and is shipped in 4°C packaging to preserve activity. TEV cleavage is best performed at room temperature in a P.H range of 4 – 9